Single-step method for labeling nucleic acids with mustard or aziridine labeling reagents
US6262252A · kind A · utility
13Cited by
1References
8Claims
0Family size
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Key dates
| Filing date | Dec 2, 1997 |
| Grant date | Jul 17, 2001 |
| Priority date | — |
| Expiry date | Dec 2, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.