Method and apparatus for rapid measurement of cell layers
US6262799A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 22, 1999 |
| Grant date | Jul 17, 2001 |
| Priority date | — |
| Expiry date | Jun 22, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/045
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Centrifuged material layer volumes are measured and quantified during centrifugation of the material layers. The material layers are differentially highlighted by means of one or more supravital fluorescent dyes or stains which are admixed with the sample being analyzed. This kinetic quantification procedure is particularly useful in performing differential blood cell and platelet counts. The blood sample is centrifuged while being subjected to an intense filtered strobed light source at two different wavelengths. At least one photometric cell layer compaction measurement is made in conjunction with the light flashes so as to record a degree of cell layer compaction as the sample is being centrifuged. In certain cases, after several successive cell layer compaction readings are derived, a compaction curve is identified and the ultimate degree of cell layer compaction is calculated from the derived compaction curve prior to the achievement of the ultimate degree of cell layer compaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.