Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6263286A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 13, 1999 |
| Grant date | Jul 17, 2001 |
| Priority date | — |
| Expiry date | Aug 13, 2019 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/086
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to methods and apparatuses for analyzing molecules, particularly polymers, and molecular complexes with extended or rod-like conformations. In particular, the methods and apparatuses are used to identify repetitive information in molecules or molecular ensembles, which is interpreted using an autocorrelation function in order to determine structural information about the molecules. The methods and apparatuses of the invention are used for, inter alia, determining the sequence of a nucleic acid, determining the degree of identity of two polymers, determining the spatial separation of specific sites within a polymer, determining the length of a polymer, and determining the velocity with which a molecule penetrates a biological membrane.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.