Extracting betulinic acid from Ziziphus jujuba
US6264998A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 1, 2000 |
| Grant date | Jul 24, 2001 |
| Priority date | — |
| Expiry date | Mar 1, 2020 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K36/725
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for isolating betulinic acid from Ziziphus jujuba is disclosed. The process involves the steps of: PA1 a) extracting bark of Ziziphus jujuba in a solvent to obtain an extract containing betulinic acid, PA1 b) semi-concentrating the extract containing betulinic acid, PA1 c) chilling said semi-concentrated extract overnight to obtain a solid in the extract, PA1 d) separating the solid from the extract by filtration or centrifugation, PA1 e) dissolving the separated solid from step d) in hot methanol, refluxing with activated charcoal and filtering through a celite bed to obtain a methanolic solution, PA1 f) partially concentrating the methanolic solution of step e), adding halogenated hydrocarbon solvent and chilling overnight to obtain a solid in the solution, PA1 g) separating the solid of step f) by filtration or centrifugation and drying the solid to obtain a solid enriched in betulinic acid, PA1 h) dissolving the dried solid step g) in a solvent containing pyridine and acetic anhydride, separating an organic layer and drying to obtain a crude 3-acetoxy betulinic acid, PA1 i) washing the crude solid 3-acetoxy betulinic acid obtained in step h) with an alcohol to yield p…
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