Detection of phosphate using coupled enzymatic reactions
US6265179A · kind A · utility
6Cited by
6References
46Claims
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Key dates
| Filing date | Feb 1, 2000 |
| Grant date | Jul 24, 2001 |
| Priority date | — |
| Expiry date | Feb 1, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Inorganic phosphate may be detected and optionally quantified via the coupling of a phosphate-dependent enzymatic reaction with an enzyme system that generates hydrogen peroxide in the presence of a chromogenic or fluorogenic peroxidase substrate. Phosphate consuming or phosphate-producing enzymes or their substrates may also be detected and/or quantified, including pyrophosphatase enzymes or pyrophosphatase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.