Compositions and methods for determining the activity of DNA-binding proteins and of initiation of transcription
US6265213A · kind A · utility
5Cited by
0References
8Claims
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Assignee
Inventors
Key dates
| Filing date | Jun 13, 2000 |
| Grant date | Jul 24, 2001 |
| Priority date | — |
| Expiry date | Jun 13, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1034
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides plasmids that are useful in detecting and determining the DNA-binding activity of sequence-specific DNA-binding molecules. The invention further provides plasmids that are useful in detecting and determining the activity of RNA polymerases in initiating transcription. In particular, the invention relates to plasmids that contain unique restriction sites and cognate nucleotide recognition sequences for sequence-specific DNA-binding molecules. Also provided are methods for using the plasmids disclosed herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.