DNA sequences coding for enzymes capable of facilitating the synthesis of linear .alpha.-1,4 glucans in plants, fungi and microorganisms

US6265635A · kind A · utility

Assignee

• PlantTec Biotechnologie GmbH Forshung & Entwicklung · DE

Inventors

• Jens Kossmann · Golm, DE
• Volker Buttcher · Lauenförde, DE
• Thomas Welsh · Lawrence, US

Key dates

• Filing date: Feb 27, 1997
• Grant date: Jul 24, 2001
• Expiry date: Feb 27, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8246
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to DNA sequences coding for proteins having the enzymatic activity of an amylosucrase which allows the synthesis of linear .alpha.-1,4 glucans from the substrate sucrose by bacteria, fungi and plants or in cell-free systems. The invention furthermore describes plasmids and bacteria containing these DNA sequences as well as processes for the production of plants and microorganisms capable of intracellularly or extracellularly expressing a polypeptide having amylosucrase activity. The invention furthermore relates to the production of pure fructose using proteins exhibiting the enzymatic activity of amylosucrase.