Gymnosperm nucleic acid molecules encoding sesquiterpene synthases and methods of use

US6265639A · kind A · utility

Assignee

• WASHINGTON STATE UNIVERSITY · US

Inventors

• Rodney B. Croteau · Pullman, US
• Jorg Bohlmann · Jena, DE
• Reinhard Jetter · Würzburg, DE
• John E. Crock · Troy, US
• Christopher L. Steele · Ardmore, US

Key dates

• Filing date: Jan 20, 1999
• Grant date: Jul 24, 2001
• Expiry date: Jan 20, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8279
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

cDNAs encoding E-.alpha.-bisabolene synthase, .delta.-selinene synthase and .gamma.-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12 and SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-.alpha.-bisabolene synthase (SEQ ID No:13), .delta.-selinene synthase (SEQ ID No:20) and .gamma.-humulene synthase (SEQ ID No: 24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-.alpha.-bisabolene synthase, .delta.-selinene synthase and .gamma.-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-.alpha.-bisabolene synthase, .delta.-selinene synthase or .gamma.-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-.alpha.-bisabolene synthase, .delta.-selinene synthase or .gamma.-humulene synthase. Thus…