Mass spectrometric methods for sequencing nucleic acids
US6268131A · kind A · utility
142Cited by
99References
34Claims
0Family size
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Key dates
| Filing date | Dec 15, 1997 |
| Grant date | Jul 31, 2001 |
| Priority date | — |
| Expiry date | Dec 15, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B60/14
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods use a modified Sanger sequencing strategy in which RNA polymerase is used to generate a set of nested RNA transcripts obtained by base-specific chain termination. These are analyzed by mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.