Purification of biologically active peptides from milk
US6268487A · kind A · utility
27Cited by
9References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 13, 1996 |
| Grant date | Jul 31, 2001 |
| Priority date | — |
| Expiry date | May 13, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/832
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of separating a soluble milk component from milk is disclosed. The method involves the use of tangential flow filtration across a membrane to form a retentate and a permeate, combining the permeate with the original milk sample, and repeating this procedure until the milk has been sufficiently purified. Preferably, the milk is combined with a chelating agent, such as EDTA, to improve the purification efficiency. This procedure is advantageously employed with milk from transgenic animals which have been genetically altered to express exogenous proteins, such as therapeutic proteins, in their milk.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.