Methods and compositions for rapid staining of nucleic acids in whole cells
US6271035A · kind A · utility
31Cited by
15References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 20, 1998 |
| Grant date | Aug 7, 2001 |
| Priority date | — |
| Expiry date | Oct 20, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/107497
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A rapid fluorescence staining method for facilitating flow cytometry analysis of reticulocytes is described. The method comprises contacting cells with a cocktail containing a detergent, sphering agent, and a cell impermeable dye, such as TO-PRO-3, for about one minute. Advantageously, the inventors have found that the cocktail permits the dye to penetrate the cell membrane rapidly.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.