Method and kit for detecting an analyte
US6271044A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 6, 1998 |
| Grant date | Aug 7, 2001 |
| Priority date | — |
| Expiry date | May 6, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25625
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a viscometric method of detecting the presence or absence of an analyte in a solution. The method utilizes a dispersion of a conjugate and a receptor. The conjugate functions as an analog of the analyte, and the receptor can interact with both the analyte and the conjugate to change the viscosity of the dispersion. The test solution is introduced into an aliquot of the dispersion. Equivalent spots of the dispersion and the aliquot containing the analyte within the dispersion are then placed on a substrate such that the spots can spread upon the substrate. The presence of analyte in the test solution is detected by noting a change in the viscosity of the dispersion through a comparison of the spots. To facilitate the method, the invention provides a test kit comprising a conjugate comprising an analog of the analyte, a receptor binding the analog and the analyte, and a substrate upon which spots of liquid can spread.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.