Single-stranded oligodeoxynucleotide mutational vectors
US6271360A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 27, 1999 |
| Grant date | Aug 7, 2001 |
| Priority date | — |
| Expiry date | Aug 27, 2019 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P43/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention concerns the introduction of predetermined genetic changes in target genes of a living cell by introducing an oligodeoxynucleotide encoding the predetermined change. The oligodeoxynucleotides are effective in mammalian, avian, plant and bacterial cells. Specific end modifications that greatly increase the effectiveness of the oligodeoxynucleotides in bacteria are described. Surprisingly, unmodified oligodeoxynucleotides can be as effective in mammaliancells, including in vivo hepatocytes, as the modified nucleotides and can be as effective or more effective than chimeric oligonucleotides that consist of a mixture of deoxynucleotides and 2'-O-methyl ribonucleotides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.