High throughput functional screening of cDNAs
US6274321A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 3, 1999 |
| Grant date | Aug 14, 2001 |
| Priority date | — |
| Expiry date | Dec 3, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1086
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for screening cDNAs that express a product interacting with a target molecule. Individual cDNAs are pooled and the cDNA pools are expressed to obtain expression products, for example by coupled in vitro transcription/translation. The interaction of the products with the target molecule is then assayed, for example by scintillation proximity assay (SPA), to identify pools of interest. By selectively re-pooling the cDNAs and repeating the expression and assay steps, individual cDNAs of interest can be rapidly identified. This method is readily automated in a computer-controlled device for high throughput screening. The invention also provides methods of transfecting a cell with a cDNA identified by the screening method to confer a desired property to a cell or identifying cDNAs from a pool of cDNAs by transfection into cells to confer a desired property.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.