Method of determining a functional linker for fusing globin subunts
US6274331A · kind A · utility
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5Claims
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Key dates
| Filing date | May 19, 1995 |
| Grant date | Aug 14, 2001 |
| Priority date | — |
| Expiry date | May 19, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/92
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A functional linker for a polypeptide in which two alpha or beta globin-like domains are genetically fused is determined by screening a library of genetically fused polypeptides, in which the linker region is varied, for the ability to participate in the formation of hemoglobin-like protein, as measured by the protein's response to carbon monoxide. In a preferred embodiment, cells expressing the protein turn red as a result of carbon monoxide pressure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.