Process and device for the isolation of cell components, such as nucleic acids, from natural sources

US6274371A · kind A · utility

Assignee

• QIAGEN GmbH · DE

Inventor

• Metin Colpan · Essen, DE

Key dates

• Filing date: Jun 19, 1997
• Grant date: Aug 14, 2001
• Expiry date: Jun 19, 2017

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/975
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A filtration process for the preparation of nucleic acids from natural sources is disclosed. The sources containing nucleic acids are lysed; the lysate is left to rest for some time; the resulting lysate passes a filter layer. The filter layer is selected from silica gel, aluminum oxide or packed diatomaceous earth, or interwoven or cemented non-wovens of glass fibers and silica gel. Other filter layers include cellulose, paper, compressed paper, and paper non-wovens. The fraction leaving the filter layer is then collected and the nucleic acid is subsequently isolated and purified from the collected fraction. Furthermore, the filter layer has not been modified with anion exchange groups.