Porphyromonas gingivalis arginine-specific proteinase coding sequences
US6274718A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 25, 2000 |
| Grant date | Aug 14, 2001 |
| Priority date | — |
| Expiry date | Jan 25, 2020 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P37/04
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Provided herein is a nucleotide sequence encoding an Arg-specific gingipain, said Arg-gingipain characterized as having an apparent molecular mass of 50 kDa as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and an apparent molecular mass of 44 kDa as estimated by gel filtration chromatography, said gingipain-1 having amidolytic and proteolytic activity for cleavage after arginine residues and having no amidolytic and/or proteolytic activity for cleavage after lysine residues, wherein the amidolytic and/or proteolytic activity is inhibited by cysteine protease group-specific inhibitors including iodoacetamide, iodoacetic acid, N-ethylmaleimide, leupeptin, antipain, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, TLCK, TPCK, p-aminobenzamidine, N-chlorosuccinamide, and chelating agents including EDTA and EGTA, wherein the amidolytic and/or proteolytic activity of said gingipain-1 is not sensitive to inhibition by human cystatin C, .alpha.2-macroglobulin, .alpha.1-proteinase inhibitor, antithrombin III, .alpha.2-antiplasmin, serine protease group-specific inhibitors including diisopropylfluorophosphate, phenylmethyl sulfonylfluoride and proteolytic activ…
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