Pressure-enhanced extraction and purification
US6274726A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 30, 1998 |
| Grant date | Aug 14, 2001 |
| Priority date | — |
| Expiry date | Jan 30, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/8813
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention is based on the discovery that hyperbaric, hydrostatic pressure reversibly alters the partitioning of biomolecules between certain adsorbed and solvated phases relative to partitioning at ambient pressure. The new methods and devices disclosed herein make use of this discovery for highly selective and efficient, low salt isolation and purification of nucleic acids from a broad range of sample types, including forensic samples, blood and other body fluids, and cultured cells. In one embodiment, the invention features a pressure-modulation apparatus. The apparatus includes an electrode array system having at least two (i.e., two, three, four, or more) electrodes; and a conduit interconnecting the electrodes. The conduit contains an electrically conductive fluid in contact with a phase positioned in a pressure chamber. The phase can be, for example, a binding medium or stationary phase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.