Regulatory sequence of cellulase cbh1 genes originating in trichoderma viride and system for mass-producing proteins or peptides therewith
US6277596A · kind A · utility
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Key dates
| Filing date | May 7, 1999 |
| Grant date | Aug 21, 2001 |
| Priority date | — |
| Expiry date | May 7, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01091
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A high-yield production system for proteins and peptides has been established, and especially a high-yield production system for cellulase in Trichoderma viride and similar filamentous fungi. The cellulase cbh1 gene regulator sequence derived from Trichoderma viride gives high expression of target proteins. The regulator sequence can therefore be used for high-yield expression of target proteins, especially cellulase. In particular, 15 g/L of an endoglucanase derived from Humicola insolens was successfully produced with this regulator sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.