Patent · US Expired

High specificity primers, amplification methods and kits

US6277607A · kind A · utility

88Cited by

10References

16Claims

0Family size

Inventors

Sanjay Tyagi · New York, US
Fred R. Kramer · Yonkers, US
Robert Vartikian · New York, US

Key dates

Filing date	May 24, 1999
Grant date	Aug 21, 2001
Priority date	—
Expiry date	May 24, 2019

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10T436/143333
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

For nucleic acid amplification including extension of primers by a DNA polymerase, high specificity primers are provided. The primers include a type of hairpin structure in which a single-stranded loop separates complementary 3' and 5' arms and in which the loop and the 3' arm are complementary to the target nucleic acid. Amplification methods, assays and kits including such primers are included in the invention.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.