Patent · US Expired

(1.fwdarw.3, 1.fwdarw.4)--.beta.-glucanase of enhanced stability

US6277615A · kind A · utility

2Cited by

0References

28Claims

0Family size

Assignees

Biomolecular Research Institute Ltd. · AU
Luminis Pty. Ltd. · AU
LA TROBE UNIVERSITY · AU

Inventors

Joseph Noozhumurry Varghese · Melbourne, AU
Thomas Peter John Garrett · Melbourne, AU
Geoffrey Bruce Fincher · Adelaide, AU
Peter Bordier Hoj · Melbourne, AU
Lin Yun Chen · Chicago, US

Key dates

Filing date	Jan 11, 1996
Grant date	Aug 21, 2001
Priority date	—
Expiry date	Jan 11, 2016

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y302/01073
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

This invention relates to a (1.fwdarw.3, 1.fwdarw.4)-.beta.-glucanase (glucanase EII endohydrolase) enzyme, whose amino acid sequence has been modified in order to provide an enzyme whose three-dimensional structure confers improved thermostability and/or pH stability. Specific modifications are based upon a comparison between the three-dimensional structure (1.fwdarw.3, 1.fwdarw.4)-.beta.-glucanase and that of (1.fwdarw.3)-.beta.-glucanase. The (1.fwdarw.3, 1.fwdarw.4)-.beta.-glucanase gene has been modified by site-directed mutagenesis, and modified enzymes have been expressed in E. coli. Modified sequences, DNA molecules encoding them, plasmids, expression vectors and transgenic plants are disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.