Preparation of factor IX

US6280729A · kind A · utility

Assignee

• Aventis Behring GmbH · DE

Inventors

• Chin C. Huang · Bourbonnais, US
• Takashi Enkoji · Park Forest, US
• Laura Ho · Bourbonnais, US
• Richard R. Kleszynski · Kankakee, US
• Richard L. Weeks · Kankakee, US
• Fred Feldman · Park Forest, US

Key dates

• Filing date: Mar 13, 2000
• Grant date: Aug 28, 2001
• Expiry date: Mar 13, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y304/21022
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A novel method of protecting blood coagulation factor IX from proteases during purification or storage is disclosed. High concentrations of one or more water soluble organic or inorganic salts are used to stabilize factor IX, contained within blood plasma-derived solutions, or contained within solutions derived from other sources, against conversion to clinically unacceptable peptide structures such as factor IX.sub.a, and/or degraded factor IX peptides. The technique is useful in stabilizing intermediate purity factor IX preparations during purification, and in maintaining the integrity of purified factor IX during long term storage. Stable high specific activity factor IX preparations are also disclosed.