Two-step hybridization and capture of a polynucleotide
US6280952A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 19, 2000 |
| Grant date | Aug 28, 2001 |
| Priority date | — |
| Expiry date | May 19, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6813
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions, which preferably differ in temperature only, is disclosed. The two hybridization conditions control the order of hybridization, where the first hybridization conditions allow hybridization of the capture probe to the target polynucleotide, and the second hybridization conditions allow hybridization of the capture probe to the immobilized probe. The method may be used to detect the presence of a target polynucleotide in a sample by detecting the captured target polynucleotide or amplified target polynucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.