Use of tyramide coating and physical separation for isolating cells or particles of interest
US6280961A · kind A · utility
1Cited by
3References
20Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 25, 1999 |
| Grant date | Aug 28, 2001 |
| Priority date | — |
| Expiry date | May 25, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56966
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to methods of tyramide coating cells or particles for physical separation, using catalyzed reporter deposition and serial amplification staining. A catalyzed reporter deposition or an analyte dependent enzyme activation system is described for detecting and/or quantitating an analyte of interest on the surface of a cell by physical separation. Also described is a method for serial amplification staining by tyramide coating cells or particles which possess an analyte of interest or a solid phase to which an analyte is bound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.