Enzyme immobilization in a gel containing 30 to 50 percent gelatin
US6280983A · kind A · utility
Inventors
Key dates
| Filing date | Feb 12, 1999 |
| Grant date | Aug 28, 2001 |
| Priority date | — |
| Expiry date | Feb 12, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Gel immobilized enzymes are prepared for use in carrying out reactions in hydrophobic solvents. The gel is formed from a gelatinizer such as gelatin or a polysaccharide such as agarose, agar, pectin, sodium alginate or carrageenan. The gel contains a ratio of amount of gelatinizer to amount of water such that the gel is capable of being mechanically divisible into dimensionally substantially stable fragments at a temperature which may reach a lower limit of the gelation temperature range of the gelatinizer. A preferred gel contains 30 to 50% gelatin, and is prepared by forming a mixture of water, enzyme and water-soluble gelatin, heating the mixture to dissolve the gelatin to form a solution, and cooling the solution until it forms a gel. An immobilized lipase can be used for synthesis of an optically active isomer of an ester from an alcohol and an acid, with either the alcohol or the acid being a racemic mixture or an enantiomer, for hydrolysis of an ester which is either a racemate or a pure enantiomer, or for transesterfication.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.