Method of detecting genetic polymorphisms using over represented sequences
US6284466A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 17, 1999 |
| Grant date | Sep 4, 2001 |
| Priority date | — |
| Expiry date | Sep 17, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2527/137
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is disclosed for the detection of polymorphisms in a DNA sequence based on nucleotide differences. DNA sequences from different sources are amplified by the polymerase chain reaction using primers based on strand biased, over represented oligonucleotide sequences and differences in the nucleotide sequences of the amplification products determined. Also disclosed are methods for the identification of individuals, or the species, strain or serotype of an organism using the method of the present invention. In addition, kits are disclosed for the determination of polymorphisms using the method of the present invention. The ability to determine genetic polymorphisms has widespread application in areas such as genome mapping, genetic linkage studies, medical diagnosis, epidemiological studies, forensics and agriculture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.