Method for extending the range of an immunoassay
US6284472A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 20, 1999 |
| Grant date | Sep 4, 2001 |
| Priority date | — |
| Expiry date | Apr 20, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/115831
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Calibrating an immunoassay by generating two reaction rate measuring curves, from samples having higher and lower relative levels of antigen, extrapolating a combination of the curves to cover sample concentrations known to contain an excess of antigen relative to an amount of capture reagent and combining the low end linear potion of the higher reaction rate measuring curve with the higher end portion of the extrapolated reaction rate measuring curve, thereby eliminating measuring inaccuracies otherwise arising from the hook effect. For antigen concentrations higher than the assay range, a high antigen signal utilizing the two rates avoids reporting false results.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.