Patent · US Expired

Enzyme with .beta.-1,3-glucanase activity

US6284509A · kind A · utility

7Cited by
1References
9Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 23, 1998
Grant dateSep 4, 2001
Priority date
Expiry dateSep 23, 2018

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY02A50/30
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

The present invention relates to a DNA construct exhibiting .beta.-1,3-glucanase activity, which DNA sequence comprises: a) the DNA sequence shown in SEQ ID No. 1 or SEQ ID No. 12, or b) an analogue of the DNA sequence defined in a), which i) is homologous with the DNA sequence defined in a), or ii) hybridizes with the same oligonucleotide probe as the DNA sequence defined in a), or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence defined in a), or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against the purified .beta.-1,3-glucanase derived from Oerksovia xanthineolytica LLG109 encoded by the DNA sequence defined in a). The DNA construct may further encode a mannose binding domain. The invention also relates to a DNA construct encoding a mannose binding domain. Further the invention relates to an expression vector comprising said DNA constructs, a cell harbouring said DNA constructs or expression vectors, a method of producing the novel enzyme, a novel enzyme preparation containing said novel enzyme, the use of the enzyme for degrading or modification of .beta.-glucan co…

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