Patent · US Expired

Class I sequence based typing of HLA-A, -B, and -C alleles by direct DNA sequencing

US6287764A · kind A · utility

13Cited by

5References

4Claims

0Family size

Inventors

William H. Hildebrand · Edmond, US
Mary Ellexson · Oklahoma City, US
Pierre Chretien · Mount Royal, CA
R. Duthie · Schenectady, US

Key dates

Filing date	Feb 11, 1998
Grant date	Sep 11, 2001
Priority date	—
Expiry date	Feb 11, 2018

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/156
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

There is provided a method for directly typing or sequencing HLA-A, -B, or -C alleles from a tissue sample wherein exons 2 and 3 of the HLA-A, -B, or -C alleles from the sample are amplified together in a locus specific manner and then separated out and individually amplified in a locus specific manner. After the two amplifications, the amplified exons are directly sequenced, the sequences are recombined, and a comparison is made between the derived HLA allele sequence and an HLA allele database, thereby giving an exact HLA-A, -B, or -C type for the sample being tested.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.