Nucleic acid promoters
US6287770A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 16, 1999 |
| Grant date | Sep 11, 2001 |
| Priority date | — |
| Expiry date | Apr 16, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method of detecting the presence of a nucleic acid target sequence of interest, the method comprising the steps of: PA1 (a) adding first and second nucleic acid probes to a sample comprising the sequence of interest, so as to form a complex comprising three strands of nucleic acid, wherein the first probe comprises the full length sequence of a first strand of a double stranded promoter, the target sequence comprises an end part of a second strand of the double stranded promoter which is complementary to a part of the first strand, and the second probe comprises the rest of the second strand of the double stranded promoter which is complementary to a part of the first strand, such that a functional promoter is formed when the first probe is hybridized to both the target sequence and to the second probe; PA1 (b) adding a polymerase which recognizes the promoter, so as to cause the de novo synthesis of nucleic acid from the promoter present in the complex; and PA1 (c) detecting directly or indirectly the de novo synthesized nucleic acid. Also disclosed is the complex formed in performance of the method defined above, and a kit for performing the method defined above.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.