Production of high titers of gibberellins, GA4 and GA7, by Gibberella fujikuroi strain LTB-1027
US6287800A · kind A · utility
Inventors
Key dates
| Filing date | Aug 23, 2000 |
| Grant date | Sep 11, 2001 |
| Priority date | — |
| Expiry date | Aug 23, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N1/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A new method of producing a mixture of gibberellins which is predominantly GA.sub.4 and GA.sub.7 but also contains GA.sub.3 has the steps of providing a seed of Gibberella fujikuroi Strain LTB-1027 or mutants derived therefrom, inoculating the seed into a culture medium rich in carbohydrate and relatively low in nitrogen, incubating the culture for at least four days, separating the Gibberella fujikuroi Strain LTB-1027 from the culture broth, and extracting the gibberellins to produce a gibberellin mixture which is at least 50% GA.sub.4 and GA.sub.7. The method produces a gibberellin mixture in which the combined titer of GA.sub.4 and GA.sub.7 exceeds 800 mg/liter. The production method also produces a gibberellin mixture with approximately equal titers of gibberellins GA.sub.4 and GA.sub.7. A variation of the method produces a gibberellin mixture which contains over 40% GA.sub.4.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.