Accessory factory function for interferon gamma and its receptor

US6287853A · kind A · utility

Assignee

• University of Medicine and Dentistry of New Jersey · US

Inventors

• Sidney Pestka · North Caldwell, US
• Serguei Kotenko · Highland Park, US
• Jaemog Soh · Highland Park, US
• Robert J. Donnelly · Leander, US
• Thomas M. Mariano · Somerville, US
• Jeffry Cook · Kendall Park, US
• Stuart Emanuel · Doylestown, US
• Barbara R. Schwartz · Annandale, US

Key dates

• Filing date: Jun 9, 1997
• Grant date: Sep 11, 2001
• Expiry date: Jun 9, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/7156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates (a) to a 540 kb YAC which encodes the necessary species-specific factor(s) and is able to substitute for human Chromosome 21 to reconstitute the Hu-IFN-gamma receptor-mediated induction of class I HLA antigens; (b) to the construction of a plasmid to integrate the selective marker for antibiotic G418 resistance into YACs and to delete some of the human DNA fragments from YACs in order to facilitate the manipulation of human genomic DNA in yeast artificial chromosome (YAC) clones; (c) to two fragmentation vectors, pSE1 and pSE2, which contain a neomycin resistance and URA3 gene, developed for targeting yeast artificial chromosomes (YACs) containing human genomic DNA; (d) to a chromosomal fragmentation procedure employed to produce a deletion set of yeast artificial chromosomes (YACs) from a parental YAC (GART D142H8) known to map to Chromosome 21q and to encode the human interferon-gamma receptor (Hu-IFN-gamma R) accessory factor gene as well as the phosphoribosylglycinamide formyltranisferase (GART) gene; and (e) to the isolation of cDNA clones that encode the necessary species-specific factor and that are able to substitute for human Chromosome 21 to reconst…