Application of .alpha.-amylase gene promoter and signal sequence in the production of recombinant proteins in transgenic plants and transgenic plant seeds
US6288302A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 4, 1998 |
| Grant date | Sep 11, 2001 |
| Priority date | — |
| Expiry date | May 4, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8234
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for producing a transgenic angiosperm plant, particularly a monocot, comprising the step of transforming an immature embryo of an angiosperm plant with a DNA fragment expressible in said angiosperm embryo, said DNA fragment comprising a promoter region derived from an .alpha.-amylase gene of a plant, and an exogenous gene encoding a desired gene product, said promoter region being inducible under a sugar-depleted or sugar-free condition to promote the expression of said gene, whereby at least a part of the resultant transgenic angiosperm plant has the ability to express said gene product. Transgenic angiosperm plants and transgenic angiosperm plant seeds are also disclosed. The transgenic angiosperm plant seeds may have application in the industrial production of alcohol, beer, glucose and the like.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.