Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US6291164A · kind A · utility
21Cited by
71References
51Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Nov 17, 1997 |
| Grant date | Sep 18, 2001 |
| Priority date | — |
| Expiry date | Nov 17, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate are disclosed. More specifically, the present invention concerns inhibiting or preventing pyrophosphorolysis in sequencing and amplification of nucleic acid molecules. According to the present invention, an enzyme which is a pentosyltransferase, a phosphotransferase with an alcohol group as acceptor, a nucleotidyltransferase, or a carboxy-lyase is added to the reaction which serves to remove pyrophosphate from the reaction mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.