Assay for determination of androgenic or anti-androgenic activity of a serum sample or a test compound
US6291194A · kind A · utility
Inventors
Key dates
| Filing date | Jul 28, 2000 |
| Grant date | Sep 18, 2001 |
| Priority date | — |
| Expiry date | Jul 28, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/723
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to methods for determining the androgenic or anti-androgenic activity of a sample and to a cell useful in these methods. To determine androgenic activity, the sample is contacted and incubated with a cell comprising PA1 i) a luciferase reporter plasmid, PA1 ii) a fusion protein comprising a ligand-binding domain of the androgen receptor and a Gal4 DNA-binding domain, said fusion protein being able to bind to binding sites in said luciferase reporter plasmid, PA1 iii) a fusion protein comprising an N-terminal region of the androgen receptor and a transcriptional activation domain, and PA1 iv) an androgen receptor-interacting protein 3. The cell is then lysed and the luciferase activity of the lysate is measured. The measured luciferase activity is compared to that obtained by repeating the above method except that a control is added instead of the sample, to give the relative luciferase activity of the sample. The relative luciferase activity is used to detect or quantify an active androgen in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.