Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
US6291205A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jun 12, 1992 |
| Grant date | Sep 18, 2001 |
| Priority date | — |
| Expiry date | Jun 12, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a process for increasing the yield of disulfide bonded recombinant proteins produced by yeast, especially recombinant secreted proteins The enzyme protein disulfide isomerase (PDI) catalyzes the formation of disulfide bonds in secretory and cell-surface proteins. We disclose the construction of recombinant strains of the yeast Saccharomyces cerevisiae which overproduce either human PDI or yeast PDI in a regulated fashion. These strains show greatly increased secretion of disulfide bonded proteins of potential therapeutic significance. These strains have the potential to increase the production of various disulfide bonded proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.