Method using an apparatus for separation of biological fluids
US6291249A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 28, 2000 |
| Grant date | Sep 18, 2001 |
| Priority date | — |
| Expiry date | Feb 28, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/2575
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A cell containing sample is separated into a cell containing portion and a substantially cell depleted portion, by mixing the sample with particles to produce a cell containing network, and separating the network from the remaining substantially cell depleted portion within a plurality of confining walls, wherein at least one of the walls is flexible. While in some aspects the separation is performed employing a magnetic force, in other aspects the separation is performed using two forces, wherein one force is a magnetic force and the other force is a mechanical force. It is contemplated that whole blood may be used as the sample, and that the cell-containing portion largely comprises a network of inter-linked red blood cells. It is especially contemplated that separation involves antiligands, preferably primary antibodies that bind to a ligand such as antigen on or in red blood cell membranes, and secondary antibodies that bind to the primary antibodies. It is also contemplated that the primary antibodies can be coupled to the surfaces of paramagnetic beads.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.