Methods for producing members of specific binding pairs
US6291650A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jun 25, 1998 |
| Grant date | Sep 18, 2001 |
| Priority date | — |
| Expiry date | Jun 25, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/735
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.