Gene expression in plants

US6294711A · kind A · utility

Assignee

• Plant Genetic Systems N.V. · BE

Inventors

• Frank Meulewaeter · Merelbeke, BE
• Marcus Cornelissen · Melle, BE
• Roel Van Aarssen · Nazareth, BE
• Piet Soetaert · Laarne, BE
• Veronique Gossele · Evergem, BE

Key dates

• Filing date: Jul 29, 1999
• Grant date: Sep 25, 2001
• Expiry date: Jul 29, 2019

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides chimeric genes comprising a first promoter recognized by DNA-dependent RNA polymerase different from a eukaryotic RNA polymerase II; a DNA region encoding a chimeric RNA comprising a 5' UTR, an AU-rich heterologous coding sequence, a 3' UTR; and optionally a terminator sequence recognized by said RNA polymerase, wherein the first promoter and the DNA region encoding the chimeric RNA are operably linked such that upon transcription by the RNA polymerase an uncapped RNA species is produced which comprises a first translation enhancing sequence derived from the 5' region of genomic or subgenomic RNA of a positive stranded RNA plant virus; a heterologous RNA coding sequence encoding a polypeptide or protein of interest, preferably from an AT-rich gene; and a second translation enhancing sequence derived from the 3' region of gonomic or subgenomic RNA of a positive-stranded RNA plant virus, wherein the uncapped RNA species is capable of being translated in the cytoplasm of a plant cell to produce the protein or polypeptide. Also provided are plant cells and plants comprising these chimeric genes, integrated in their nuclear DNA, whereby the plant cell produces the…