Methods and apparatus for detecting nucleic acid polymorphisms

US6297018A · kind A · utility

Assignee

• LJL Biosystems, Inc. · US

Inventors

• Todd French · Mountain View, US
• Douglas N. Modlin · San Mateo, US
• John C. Owicki · Palo Alto, US
• James S. Richey · Palo Alto, US
• Lev J. Leytes · Palo Alto, US
• Enal S. Razvi · San Francisco, US

Key dates

• Filing date: Jan 28, 2000
• Grant date: Oct 2, 2001
• Expiry date: Jan 28, 2020

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/9121
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) allele-specific hybridization, using luminescence detection, (2) allele-specific oligonucleotide ligation, using dye-labeled oligonucleotide ligation with luminescence resonance energy transfer (FRET) detection, and (3) allele-specific nucleotide incorporation, using primer extension with luminescence polarization (FP) detection. More specifically, the methods may include (1) locating a sample containing a nucleic acid material at an examination site, (2) illuminating the sample, (3) detecting light transmitted from the sample, and (4) determining the presence, absence, and/or identity of a nucleic acid target in the sample using the light transmitted from the sample.