Uronium salts for activating hydroxyls, carboxyls, and polysaccharides, and conjugate vaccines, immunogens, and other useful immunological reagents produced using uronium salts
US6299881A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 23, 1998 |
| Grant date | Oct 9, 2001 |
| Priority date | — |
| Expiry date | Mar 23, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/806
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for producing a conjugate vaccine includes mixing a uronium salt reagent with a first moiety (e.g., a polysaccharide). According to the invention, the uronium salt reagent has a chemical structure corresponding to formula I: ##STR1## wherein R.sup.1 is defined as ##STR2## wherein R.sup.6 represents the carbon, hydrogen, and optionally one or more heteroatoms which, together with the nitrogen atom to which they are attached, constitute a 5 to 10 membered heterocyclic ring, which may be substituted or unsubstituted. R.sup.2, R.sup.3, R.sup.4, and R.sup.5, each independently represents a hydrogen atom, a substituted or unsubstituted alkyl having 1 to 6 carbon atoms, a substituted or unsubstituted alkenyl having 2 to 6 carbon atoms, or an alkynyl having 2 to 6 carbon atoms. Alternatively, R.sup.2 and R.sup.3, when taken together, can represent the carbon, hydrogen, sulfur, nitrogen, or oxygen atoms necessary to complete a 5 to 7 membered heterocyclic ring with the nitrogen atom to which they are attached. Likewise, R.sup.4 and R.sup.5, when taken together, can represent a similar heterocyclic ring. X.sup.- represents an acid anion (e.g., Cl.sup.-, Br.sup.-, F.sup.-, I.sup.-, P…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.