Method for measuring messenger RNA
US6300058A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Nov 12, 1992 |
| Grant date | Oct 9, 2001 |
| Priority date | — |
| Expiry date | Nov 12, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/158
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for detecting and quantifying mRNA in a sample. The mRNA that can be detected has a unique sequence. The method includes immobilizing a first polynucleotide to an insoluble support. The first polynucleotide has a first sequence that hybridizes to the unique sequence on the mRNA. After immobilization of the first polynucleotide, the sample is applied to the insoluble support under conditions that allow the unique sequence on the mRNA to hybridize with the first polynucleotide. Thereafter, a second polynucleotide is applied to the insoluble support. This second polynucleotide has a second sequence thereon that hybridizes to a portion of the mRNA other than the unique sequence. The application of the second polynucleotide is performed under conditions that allow the second polynucleotide to hybridize with mRNA immobilized on said support, if present. Finally, the amount of the second polynucleotide immobilized on the support is measured to provide an indication of the amount of mRNA present in the sample. Polynucleotide immobilized supports and sequences useful in the method are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.