Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
US6300075A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 28, 2000 |
| Grant date | Oct 9, 2001 |
| Priority date | — |
| Expiry date | Jan 28, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are improved methods for amplifying nucleic acids. The methods encompass a method for increasing the specificity of amplification of a target nucleic acid in an amplification reaction, where the reaction reagents include one or more oligonucleotide amplification primers specific to the target nucleic acid, a target nucleic acid, a nucleic acid polymerase, and one or more magnesium salts, by preparing a primer/carrier mixture comprising one or more oligonucleotide amplification primers and carrier nucleic acid, and contacting the primer/carrier admixture with target nucleic acid, one or more magnesium salts, and nucleic acid polymerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.