Pullulanase expression constructs containing .alpha.-amylase promoter and leader sequences
US6300115A · kind A · utility
4Cited by
10References
25Claims
0Family size
Assignee
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Key dates
| Filing date | May 18, 1999 |
| Grant date | Oct 9, 2001 |
| Priority date | — |
| Expiry date | May 18, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01041
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are DNA expression constructs containing an .alpha.-amylase promoter sequence derived from Bacillus stearothermophilus, an .alpha.-amylase leader sequence derived from Bacillus stearothermophilus, and a DNA sequence encoding a pullulanase derived from Bacillus naganoensis. Microbial hosts transformed to contain the expression constructs secret function pullulanases. Also disclosed is a process for making recombinant pullulanases utilizing the expression constructs and a recombinant pullulanase which can be produced in Bacillus subtilis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.