Card-based biosensor device
US6300141A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 2, 2000 |
| Grant date | Oct 9, 2001 |
| Priority date | — |
| Expiry date | Mar 2, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54366
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A diagnostic card device for use in detecting or quantitating an analyte present in a liquid sample, comprising a card substrate having a sample introduction region, a biosensor, and a sample-flow pathway communicating between the sample-introduction region and the biosensor, circuitry for generating an analyte-dependent electrical signal from the biosensor; and a signal-responsive element for recording such signal. In one embodiment, the biosensor includes a detection surface with surface-bound molecules of a first charged, coil-forming peptide capable of interacting with a second, oppositely charged coil-forming peptide to form a stable .alpha.-helical coiled-coil heterodimer, where the binding of the second peptide to the first peptide, to form such heterodimer, is effective to measurably alter a signal generated by the biosensor. The sample-flow pathway contains diffusibly bound conjugate of the second coil-forming peptide and the analyte (or an analyte analog) and immobilized analyte-binding agent. The analyte in the liquid sample and the conjugate compete for binding with the immobilized analyte-binding agent. Unbound conjugate migrates by capillarity to the biosensor. Liquid…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.