Functional characterization of genes
US6300542A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 24, 1999 |
| Grant date | Oct 9, 2001 |
| Priority date | — |
| Expiry date | May 24, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6895
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Insertions into a gene of known sequence can be generated by crossing two parent plants, one of which contains a transposable element, to produce F.sub.1 progeny plants in which the insertion is detected by means of a PCR. F.sub.1 progeny plants containing such an insertion are self-fertilized to produce F.sub.2 progeny which are homozygous for the insertion. The function of a gene disabled by the insertion can be ascertained from a comparison of the phenotype of the F.sub.2 progeny with a parental phenotype. Large numbers of F.sub.1 progeny can be tested simultaneously for the presence of insertions. A collection of F.sub.2 seed can be stored and used for phenotype comparison when an insertion is detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.