Method for attachment of biomolecules to surfaces through amine-functional groups
US6303179A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 8, 1999 |
| Grant date | Oct 16, 2001 |
| Priority date | — |
| Expiry date | Feb 8, 2019 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61L33/0029
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
A method of modifying the surface characteristics of a substrate having a surface with an amide-functional polymer thereon. The method involves contacting the amide-functional polymer with a source of hydroxide ions and a source of hypohalite ions at a temperature of at least about 20.degree. C. for a time effective to convert at least a portion of the amide-functional groups to amine-functional groups to form a substrate surface comprising an amine-functional polymer, wherein the hydroxide ions are present in a molar excess relative to the hypohalite ions and at a concentration of no more than about 0.1 M, based on the total volume of the reaction mixture. A biomolecule is attached to the resultant amine-functional polymer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.