Patent · US Expired

Cloning vectors and their preparation and use for mRNA expression pattern analysis

US6303308A · kind A · utility

5Cited by

9References

21Claims

0Family size

Assignee

Switch Biotech AG · DE

Inventors

Jorn-Peter Halle · Penzberg, DE
Johannes Regenbogen · Martinsried, DE
Andreas Goppelt · Hersbruck, DE

Key dates

Filing date	May 10, 1999
Grant date	Oct 16, 2001
Priority date	—
Expiry date	May 10, 2019

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/1096
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed herein are cloning vectors which include: PA1 (a) a cloning site which permits the cloning of a nucleic acid in defined orientation; PA1 (b) at least one cleavage site adjacent to the cloning site, the cleavage site being rarely-occurring in nucleic acids; and PA1 (c) a long region which is located on the side of the cloning site opposite to the cleavage site (b), wherein the long region and the region between the cloning site and the cleavage site (b) contain neither the cloning site nor at least two frequently-occurring cleavage sites.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.