Method for producing a peptide with a pI above 8 or below 5
US6303340A · kind A · utility
3Cited by
4References
27Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 8, 1999 |
| Grant date | Oct 16, 2001 |
| Priority date | — |
| Expiry date | Jul 8, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is described for producing a peptide having a pI above 8 or below 5 wherein the peptide is expressed as a fusion protein in which it is linked to a fusion partner through an acid cleavage site. The peptide is released from the fusion partner by acid cleavage in the absence of chaotrope. The fusion partner and its acid cleavage products, if any, have a net charge sufficiently different from that of the desired peptide to allow isolation of the peptide by ion-exchange chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.