Patent · US Expired

Methods and compositions for polypeptide engineering

US6303344A · kind A · utility

201Cited by
55References
12Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 24, 1999
Grant dateOct 16, 2001
Priority date
Expiry dateJun 24, 2019

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC40B50/06
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

R): A25. 28.sup.th Annual Meeting of the Swiss Societies for Experimental Biology. Mar. 27-29, 1996. Carter, Paul (1985) "Improved Oligonucleotide-Directed Mutagenesis Using M13 Vectors" Annu. Rev. Genet. Methods in Enzymology vol. 154 pp 382-403. Kramer & Fritz (1987) "Oligonucleotide-Directed Construction of Mutations via Gapped Duplex DNA" Methods in Enzymology vol. 154 pp 350-367. Kunkel, Thomas A. (1985) "Rapid and efficient site-specific mutagenesis without phenotypic selection" Proc. Matl. Acad. Sci. USA vol. 82 pp. 488-492. Zoller & Smith (1987) "Oligonucleotide-Directed Mutagenesis: A Simple Method using Two Oligonucleotide Primers and a Single-Stranded DNA Template" Methods in Enzymology vol. 154 pp 329-347. Delgrave et al., (1993) Bio/Technology 11:1548-1552. Goldman, E.R. and Youvan D.C., (1992) Bio/Technology 10:1557-1561. Joyce, G.F., (1992) Scientific American, Dec., 90-97. Bock, L.C., et al., (1992) Nature 355:564-566. Scott, J.K. and Smith G.P., (1990) Science 249:386-390. Cwirla, S.E. et al.,(1990) Proc Nat'l Acad Sci USA 87:6378-6382. McCafferty, J. et al., (1990) Nature 348:552-554. Cull, M.G. et al.,(1992) Proc Natl Acad Sci USA 89:1865-1869. Nissim et al., (19…

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